SRA

The experiment aimed to (1) identify SNPs that affect a male's success in sexual selection; (2) test if these SNPs are different or differentially affected depending on whether or not the males were exposed to a pathogen prior to assessment of their success in sexual selection; and (3) assess to what degree SNP variants favored by sexual selection overlap with variants that promote survival of infection by males and females. Drosophila melanogaster males from an outbred laboratory population were subject to oral infection with Pseudomonas entomophila at a high dose (OD600 = 100), low dose (OD600 = 20) or sham treatment (buffer). Subsequently, the males were subject to mating contests where two males (from the same treatment) were paired with a virgin female; male that mated first was identified as the "winner" and the other male as the "loser". The detailed protocol was the same as in https://doi.org/10.1098/rspb.2019.0226 . 140 "winner" and 140 "loser" males from each infection/sham treatment were collected this way, split haphazardly into two pools of 70 and subject to DNA extraction and pool sequencing on Illumina HiSeq (samples 1-6 and 11-16).In a separate experiment, virgin males and females were exposed to P. entomophila infection (males at OD600 = 100, females at OD600 = 50 because of their greater susceptibility) or to the sham treatment. After 72 h the mortality of infected females reached 50% and of the males 30%; at this point the survivors were collected for sequencing. At the same time males and females from the sham treatment were likewise collected for sequencing. Two pools of 70 flies were collected for each sex and infection treatment and sequenced as above (samples 7-10 and 17-20).